DNBSEQ-G99 High-throughput Sequencing Set
DNBSEQ-G99 High-throughput Sequencing Set is developed based on MGI's core DNBSEQ ™ sequencing technology. Novel designed reagent cartridge applies to the sequencing sets. Supporting oncology panel, exome, small genome sequencing and other applications. By adopting the DNBSEQ ™ technology with the innovative StandardMPS 2.0 sequencing reagents, this upgrade delivers an impressive proportion of 85% or higher for base quality scores reaching or exceeding Q40 during the sequencing process.
Product Specification
| Maximum number of Flow Cells | Lanes/Flow Cell | Flow Cell Type | Reads/Flow Cell* | Supported Read Lengths | Data Output | Q30*** | Q40*** | Run Time*** |
|---|---|---|---|---|---|---|---|---|
| 2 | 1 | PCS | 40 M | App-D PE150 | 12 Gb~24 Gb | >90% | >85% | ~11 h |
| FCL | 80 M | SE100/PE50 | 8 Gb~16 Gb | >90% | >85% | ~5 h | ||
| PE150 | 24 Gb~48 Gb | >90% | >85% | ~12 h | ||||
| App-D SE100 | 8 Gb~16 Gb | >90% | >85% | ~5 h | ||||
| App-D PE150 | 24 Gb~48 Gb | >85% | >85% | ~12 h | ||||
| FCU | 200 M | App-D PE300 | 48 Gb~96 Gb | >85% | >85% | ~30 h | ||
| SE400 | 32 Gb~64 Gb | >75% | >70% | ~20 h | ||||
| 2 | 1 | FCU | 200 M | App-D SE100 | 20 Gb~40 Gb | >90% | >85% | ~7 h |
| App-D PE150 | 60 Gb~120 Gb | >85% | >85% | ~16 h | ||||
| App-D PE300 | 120 Gb~240 Gb | >85% | >80% | ~35 h |
* Effective reads is determined using a standard library. Actual output may vary depending on sample type and library preparation method.
** The percentage of bases above Q30 and run time is the average of an internal standard library over the entire run. The actual performance is affected by factors such as sample type, library quality, and insert fragment length. Only StandardMPS 2.0 (SM 2.0) reagents support the generation of Q40 data.
*** The sequencing time is the statistical duration for single flow cell sequencing and dual flow cell simultaneous sequencing.
★ The instrument is equipped with SE50 and PE100 sequencing modes, and the existing reagent kits support SE50 and PE100 read length sequencing.
Note: APP-D has built-in Illumina's Truseq, Nextera adapters, and MGI adapter, which supporting mixed testing of Illumina's Truseq, Nextera adapters, and MGI adapter libraries.