DNBSEQ-G99
8 Gb ~ 240 Gb per run
Only 12 hrs for PE150 (from loading to FASTQ)
Supports multiple read lengths, including PE50, SE100, PE150, PE300, SE400
Applicable to multiple sequencing methods
As one of the sequencers with the fastest speed among mid-low throughput in the world, DNBSEQ-G99 (1) is developed based on MGI's core DNBSEQ™ sequencing technology, enabled by innovations in biochemistry, optics, fluidics, temperature control, and other core systems. DNBSEQ-G99 is especially applicable for multiple sequencing methods, it only needs 12 hours to finish PE150 sequencing with Q40 data. Different types of flow cells can be selected for sequencing according to the sample quantity.
| Maximum number of Flow Cells | Lanes/Flow Cell | Flow Cell Type | Reads/Flow Cell* | Supported Read Lengths | Data Output | Q30*** | Q40*** | Run Time*** |
|---|---|---|---|---|---|---|---|---|
| 2 | 1 | FCS | 40 M | App-PE150 | 12 Gb~24 Gb | >90% | >85% | ~11 h |
| FCL | 80 M | SE100/PE50 | 8 Gb~16 Gb | >90% | >85% | ~5 h | ||
| PE150 | 24 Gb~48 Gb | >90% | >85% | ~12 h | ||||
| App-DE100 | 8 Gb~16 Gb | >90% | >85% | ~5 h | ||||
| App-PE150 | 24 Gb~48 Gb | >85% | >85% | ~12 h | ||||
| App-PE300 | 48 Gb~96 Gb | >85% | >85% | ~30 h | ||||
| SE400 | 32 Gb~64 Gb | >75% | >70% | ~20 h | ||||
| FCU | 200 M | App-DE100 | 20 Gb~40 Gb | >90% | >85% | ~7 h | ||
| App-PE150 | 60 Gb~120 Gb | >85% | >85% | ~16 h | ||||
| App-PE300 | 120 Gb~240 Gb | >85% | >80% | ~35 h | ||||
|
* Effective reads is determined using a standard library. Actual output may vary depending on sample type and library preparation method. ** The percentage of bases above Q30 and run time is the average of an internal standard library over the entire run. The actual performance is affected by factors such as sample type, library quality, and insert fragment length. Only StandardMPS 2.0(SM 2.0) reagents support the generation of Q40 data. *** The sequencing time is the statistical duration for single flow cell sequencing and dual flow cell simultaneous sequencing. ★ The instrument is equipped with SE50 and PE100 sequencing modes, and the existing reagent kits support SE50 and PE100 read length sequencing. Note: APP-D has built-in Illumina’s Truseq, Nextera adapters, and MGI adapter, which supporting mixed testing of Illumina’s Truseq, Nextera adapters, and MGI adapter libraries. |
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| Method | Application | Recommended read length | Recommended data size | FCS | FCL | FCU |
|---|---|---|---|---|---|---|
| Recommended sample /RUN | ||||||
| Target capture/multiplex PCR | Onco panel | PE100/PE150 | Small panel: ~1 Gb/sample | 12/FC,24/RUN | 24/FC,48/RUN | 60/FC,120/RUN |
| Genetic disease diagnosis small panel (Thalassemia,deafness, etc.) | PE150 | Deafness: ~5 Gb/sample | 2/FC,4/RUN | 4/FC,8/RUN | 12/FC,24/RUN | |
| Thalassemia：~0.2M reads/sample | 200/FC,400/RUN | 400/FC, 800/RUN | 1000/FC, 2000/RUN | |||
| ATOPlex panel (Respiratory, COVID-19, etc.) | PE100/PE150 | Respiratory panel: 5M reads/sample COVID-19 panel: 5M reads/sample | 8/FC,16/RUN | 16/FC,32/RUN | 40/FC,80/RUN | |
| WES | PE150 | 15 Gb/sample | / | 1-2/FC,2-4/RUN | 4/FC,8/RUN | |
| Methylation | Onco targeted methylation panel | PE150 | ~5 Gb/sample | 2/FC,4/RUN | 4/FC,8/RUN | 12/FC,24/RUN |
| Small genome sequencing | Metagenomics for pathogen detection | SE50/SE100 | Meta:20 M reads/sample | / | 4/FC,8/RUN | 10/FC,20/RUN |
| Microbial WGS sequencing | PE100/PE150 | Single bacterium: ~1 Gb/sample | 12/FC,24/RUN | 24/FC,48/RUN | 60/FC,120/RUN | |
| 16S sequencing | PE300 | ~0.1 M reads/sample | / | 640/FC,1280/RUN | 1280/FC,2560/RUN | |
| Low-pass WGS sequencing | NIPT | SE50 | NIPT/PGS:~10 M reads/sample | / | 8/FC,16/RUN | 20/FC,40/RUN |
| PGS | SE50 | / | ||||
| Transcriptome sequencing | RNA-Seq | SE50/PE150 | Expression profiling:~25M/sample | / | 3/FC,6/RUN | 8/FC,16/RUN |
| Transcriptome: ~6 Gb/sample | / | 4/FC,8/RUN | 10/FC,20/RUN | |||
| Forensics | DNA signature identification | SE400 | ~0.8 M reads/sample | / | 96/FC,192/RUN | / |